Laboratory of Genomics and Gene Expression in Cancer
Identification of molecular markers and therapeutic targets in pancreatic cancer
In our laboratory we employ genomic methods (DNA microarray hybridization, high throughput RNA sequencing) to analyze global gene expression profiles from clinical samples of pancreatic adenocarcinoma aiming at identifying changes in expression of protein-coding and noncoding RNAs (ncRNAs) that correlate with the malignant transformation or tumor progression.
A recent focus of interest of our lab is to apply these approaches to the analysis of cellular and animal models that recapitulate disease progression in patients thus facilitating the identification of novel therapeutic targets and genes involved in resistance to conventional chemotherapy.
Functional characterization of long noncoding RNAs
Recent studies have shown that a large fraction of the eukaryotic transcriptome is comprised of RNAs without protein-coding potential that map to intronic and intergenic regions. We investigate in our lab the biological roles and mechanism of action of long (> 200nt) ncRNAs in gene expression regulation in human tissues. To that end, we are currently investigating the biogenesis, processing and sub-cellular localization of long ncRNAs, and evaluate the phenotypic effects induced by overexpression/silencing of these transcripts in different biological models.