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Fotografia: Maurício da Silva Baptista
Maurício da Silva Baptista
Professor Titular

Departamento de Bioquímica
Instituto de Química - Universidade de São Paulo

E-mail: baptista@iq.usp.br
Tel: +55 11 3091-8952

Formação Acadêmica:
Pharmacy & Biochemistry, University of São Paulo, São Paulo, Brazil 1986-1990
M.S. Biochemistry, University of São Paulo, São Paulo, Brazil 1990-1992
Ph.D. Chemistry, Marquette University, Milwaukee, WI 1992-1996
Pos-doc Photobiology, University of Wisconsin-Madison, WI 1996-1997

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Linha de Pesquisa: FOTOSSENSIBILIZAÇÃO NAS CIÊNCIAS DA VIDA (PHOTOSENSITIZATION IN LIFE SCIENCES)

Resumo:

Fotossensibilização ocorre quando compostos fotoativos absorvem luz e a transferem para moléculas vizinhas, de tal forma que a energia luminosa é transformada em química. A natureza utiliza os processos de fotossensibilização de forma organizada e controlada. No entanto, estes processos podem ficar descontrolados, gerando espécies reativas, que causam danos em células em tecidos, por exemplo, na pele do ser humano que se expõem demasiadamente ao sol. O homem aprendeu a utilizar esta geração foto-induzida de espécies excitadas e reativas para destruir células e tecidos doentes e consequentemente tratar diversas doenças, em um processo denominado Terapia Fotodinânica (PDT, do inglês Photodynamic Therapy). Nesta linha de pesquisa pretendemos entender no nível molecular os mecanismos de fotossensibilização que atuam durante a PDT ou durante a exposição exagerada ao sol, utilizando diversos modelos de estudo: os ambientes e técnicas computacionais, os sólidos e as soluções de compostos e nanomateriais purificados, os modelos biomiméticos, as células em cultura, modelos animais e estudos clínicos em pacientes. Estes modelos de estudo são normalmente utilizados/dominados por pesquisadores com especialidades em disciplinas distintas, ou seja, física, química, biologia, bioquímica, farmácia e medicina, que neste projeto formarão uma equipe para tratar o tema das reações de fotossensibilização de maneira multidisciplinar, formando um ambiente ideal para o treinamento de graduandos, pós-graduandos, pós-doutores e jovens pesquisadores. Além disso, a colaboração com instituições clínicas permitirá que alguns destes conhecimentos sejam rapidamente aplicados na prática clínica trazendo benefícios imediatos para a sociedade. Para alcançar estes objetivos dividimos este projeto em quatro sub-projetos, que são: estudo dos mecanismos da PDT, síntese de novos fotossensibilizadores para PDT, caracterização dos mecanismos de fotossensibilização induzidos por luz solar UVA e visivel, resposta aos processos de fotossensibilização em animais e humanos.
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Summary

Photosensitization occurs when photoactive compounds absorb light and transfer it to neighboring molecules, such that light energy is transformed into chemical. Nature uses the processes of photosensitization in an organized and controlled way. However, due to several factors, these processes can become uncontrolled, generating reactive species that cause damage to cells in tissues, i.e., skin of humans who are over exposed to the sun. Man learned to use this photo-induced generation of excited and reactive species to destroy cells and tissues and consequently to treat several diseases, in a process called Photodynamic Therapy (PDT). Our research goal is to understand, at the molecular level, the mechanisms of photosensitization that take place during PDT or during exaggerate exposure of animals and plants to the sun, using different systems of study: computational techniques, purified compounds and nanomaterials in solid phase and in isotropic solutions, biomimetic models and cell cultures. These models are usually used/dominated by researchers with distinct expertises working in different disciplines, namely physics, chemistry, biology, biochemistry, pharmacy and medicine. In this research line we will work as a team to address the issue of photosensitization in a multidisciplinary manner, which is the ideal environment for training undergraduates, graduate and postdoctoral fellows and young researchers. In addition, collaboration with medical institutions allows that some of this knowledge is rapidly applied in clinical practice, bringing immediate benefits to the society. To achieve this overall goal we have identified four sub-projects which are: study of the PDT mechanisms, synthesis of new photosensitizer agents for PDT, characterization of the mechanisms of photosensitization induced by UVA and visible sun light, case studies in animals and humans.


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resumo_visual: Maurício da Silva Baptista


Produção científica:

Curriculo (Sistema Lattes - CNPq)


20 de ~100

1. Baptista, M.S.; Indig, G.L. Effect of BSA Binding on Photophysical Photochemical Properties of Triarylmethane Dyes The Journal of Physical Chemistry B 1998, 102, 4678-4688.
2. Junqueira, H. C.; Severino, D.; Dias, L.G. ; Gugliotti, M. ; Baptista, M. S. “Modulation of the Methylene Blue Photochemical Properties Based on the Adsorption at Aqueous Micelle Interfaces” Phys.Chem.Chem.Phys. 2002,4, 2320.
3. Severino,D.; Junqueira,H.C.; Gabrielli, D.S., Gugliotti, M., Baptista,M.S. “Influence of Negatively Charged Interfaces on the Ground and Excited State Properties of Methylene Blue” Photochem.Photobiol. 2003, 77, 459.
4. Gabrieli,D.; Belisle,E.; Severino,D.; Kowaltowski,A.J.; Baptista,M.S. “Binding, aggregation and photochemical properties of methylene blue in mitochondrial suspensions” Photochem.Photobiol. 2004, 79, 227.
5. Tumolo, T., Angnes,L:, Baptista, M. S., Determination of the Refractive Index Increment (dn/dc) of molecule and macromolecule solutions by Surface Plasmon Resonance, Anal. Biochem., 2004, 333, 273-279.
6. Tardivo, J.P.; Del Giglio, A.; Oliveira, C.S.; Gabrielli, D.S.; Junqueira, H.C.; Tada, D.B.; Severino, D.; Turchiello, R., Baptista, MS. Methylene Blue in Photodynamic Therapy: From Basic Mechanisms to Clinical Applications, Photodyagnosis and Photodynamic Therapy 2005, 2/3, 175-191. (74)
7. Suraniti,E.; Tumolo,T.; Baptista,M.S.; Livache,T.; Calemczuk,R. Construction of hybrid bilayer membrane (HBM) biochips and characterization of the cooperative binding between cytochrome-c and HBM Langmuir 2007, 23, 6835-6842.
8. Tada, D.; Vono,L.L.R.; Duarte,E.Itri,R.; Kiyohara,P.K.; Baptista,M.S.; Rossi,L.M. Methylene blue-containing silica coated magnetic particles: a potential magnetic carrier for photodynamic therapy, Langmuir 2007, 23, 8194-8199.
9. Pavani, C.; Uchoa,A.F.; Oliveira, C.S; Iamamoto, Y.; Baptista,M.S. Effect of zinc insertion and hydrophobicity on the membrane interactions and PDT activity of porphyrin photosensitizers Photochem.Photobiol.Sci. 2009, 8, 233.
10. Barros, Teresa C., Toma, Sergio H., Toma, Henrique E., Bastos, Erick L., Baptista, Mauricio S. Polymethine cyanine dyes in -cyclodextrin solution: multiple equilibria and chemical oxidation. Journal of Physical Organic Chemistry. , v.Mar26, p.n/a - n/a, 2010.
11. Uchoa, Adjaci F., Oliveira, Carla S., Baptista, Mauricio S. Relationship between structure and photoactivity of porphyrins derived from protoporphyrin IX. Journal of Porphyrins and Phthalocyanines. , v.14, p.832 - 844, 2010.
12. Chiarelli-Neto O; Pavani, C.; Ferreira A.S.; Uchoa, A.; Severino, D.; Baptista,M.S. Generation and suppression of singlet oxygen in hairs by photosensitization of melanin. Free Radic. Biol. Med. 2011, 51, 1195–1202.
13. Oliveira, Carla S., Turchiello, Rozane, Kowaltowski, Alicia J., Indig, Guilherme L., Baptista, Mauricio S. Major determinants of photoinduced cell death: Subcellular localization versus photosensitization efficiency. Free Radical Biology & Medicine. , v.51, p.824 - 833, 2011.
14. Baptista, M.S., Wainwright, M. Photodynamic antimicrobial chemotherapy (PACT) for the treatment of malaria, leishmaniasis and trypanosomiasis. Brazilian Journal of Medical and Biological Research (Impresso). , v.44, p.1 - 10, 2011.
15. Silva, Paulo R., Vono, Lucas L. R., Espósito, Breno P., Baptista, Maurício S., Rossi, Liane M. Enhancement of hematoporphyrin IX potential for photodynamic therapy by entrapment in silica nanospheres. PCCP. Physical Chemistry Chemical Physics (Print). , v.13, p.14946 - 14952, 2011.
16. Baptista, Maurício S., Pavani, Christiane, Iamamoto, Yassuko Mechanism and Efficiency of Cell Death of Type II Photosensitizers: Effect of Zinc Chelation¿. Photochemistry and Photobiology. , v.88, p.774-781, 2012.
17. Haluska, Christopher K., Baptista, Mauricio S., Fernandes, Adjaci U., Schroder, André P., Marques, Carlos M., Itri, Rosangela Photo-activated phase separation in giant vesicles made from different lipid mixtures. Biochimica et Biophysica Acta. Biomembranes. , v.1818, p.666 - 672, 2012.
18. Cordeiro, R. M., Miotto, R., Baptista, M.S., Photodynamic Efficiency of Cationic -Porphyrins at Lipid Bilayers: Insights from Molecular Dynamics Simulations. The Journal of Physical Chemistry. B (1997 : Online). , v.116, p.14618 - 14627, 2012.
19. Martins, W. K., Severino, Divinomar, SOUZA, C., STOLF, B. S., Baptista, Mauricio S.
A novel in vitro approach for rapid screening of potential autophagic inductor agents using mammalian cell lines. Biotechnology Journal (Print). , v.8, p.n/a - n/a, 2013.
20. Deda, Daiana K., Pavani, C., Carita, E., Baptista, M.S., Toma, Henrique E., Araki, Koiti. Control of Cytolocalization and Mechanism of Cell Death by Encapsulation of a Photosensitizer. Journal of Biomedical Nanotechnology.2013, in press.

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